2014 Cruise Data and Laboratory Protocols

Data from the 2014 Kilo Moana cruise
(Monday, June 09 thru Monday, June 16

Please note: As these data sets and protocols become available after the cruise, access will be restricted to Summer Course participants and login will be required. Mahalo!

Data Sets

Cruise Binder (PDF)
CTD Data (FTP)
CTD Plots (PDF)
Autoanalyzer nutrients (.xlsx)
Underway Data (FTP)
Optics Data (FTP)
Surface PAR Plot (PDF)
Water column particulate phosphorus (.xls)
Sediment trap particulate phosphorus (.xls)
Fluorometric chlorophyll (.xls)
Sediment trap particulate silica (.xlsx)
Bacterial production (.xlsx)
Spectrophotometric silicate (.xlsx)
16S phylotypes (.xlsx)
Primary productivity array (.xlsx)
Low level phosphate (.xlsx)
Low level nitrate+nitrite (.xlsx)
Flow cytometric cell counts (.xlsx)
Fast repetition rate fluorometry (.txt)
3H-leucine photosynthetron (.xlsx)
14C-photosynthetron (.xls)
Water column PC/PN (.xls)
Sediment PC/PN (.xls)

Laboratory Protocols

High sensitivity nitrate concentrations (Word)
Determination of particulate phosphorus concentrations (Word)
Measurement of primary production (Word)
Measurement of bacterial production (Word)
ATP concentrations (Word)
Silicic acid concentrations (Word)
Phosphate concentrations (Word)
Diversity Group Protocols (PDF)
CMORE Summer 2014 Diversity Samples (.xlsx)
Boström, et al., 2004. “Optimization of DNA extraction for quantitative marine bacterioplankton community analysis” (PDF)
Brody & Kern, 2004. “History and principles of conductive media for standard DNA electrophoresis” (PDF)
Brody, et al., 2004. “Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media” (PDF)
Caporaso, et al., 2012. “Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms” (PDF)
Miller, et al., 1988. “A simple salting out procedure for extracting DNA from human nucleated cells” (PDF)
Mueller, et al., 2014. “Variables Influencing Extraction of Nucleic Acids from Microbial Plankton (Viruses, Bacteria, and Protists) Collected on Nanoporous Aluminum Oxide Filters” (PDF)
Steward & Culley, 2010. “Extraction and purification of nucleic acids from viruses” (PDF)
Watson, et al., 1998 “A New Method for DNA and RNA Purification” (PDF)
New! Chavez, et al., 19985. “On the Chlorophyll a Retention Properties of Glass-Fiber GF/F Filters” (PDF)
New! Karl, 2007. “Plastics-Irradiated-Etched: The Nuclepore® Filter Turns 45 Years Old” (PDF)
New! “Membrane filtration: A User’s Reference” and “Polycarbonatemembrane filters” (PDF)
New! Burford and Pollard, 1994. “Pigment contaminants in polycarbonate filters” (PDF)

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2014 Cruise Data and Laboratory Protocols

Data from the 2014 Kilo Moana cruise (Monday, June 09 thru Monday, June 16

Data Sets

Laboratory Protocols

Data from the 2014 Kilo Moana cruise
(Monday, June 09 thru Monday, June 16