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C-MORE Home | Education | Summer Course Home | 2015 Summer Course | 2015 Data & Protocols

2015 Cruise Data and Laboratory Protocols

Data from the 2015 Kilo Moana cruise
Wednesday, June 10 – Wednesday, June 17

Please note: As these data sets and protocols become available after the cruise, access will be restricted to Summer Course participants and login will be required. Mahalo!

Data Sets

• CTD Data (FTP)
• CTD Plots (PDF)
• Underway Data (FTP)
• Optics Data (FTP)
• Surface PAR Plot (PDF)
• Inorganic Nutrients (.xlsx)
• Bacterial production - arrays and photosynthetrons (.xlsx)
• Particulate phosphorus (.xlsx)
• Dissolved silicate (.xlsm)
• ATP concentrations (.xlsx)
• Chlorophyll concentrations (.xlsx)
• Low level phosphorus concentrations (.xlsx)
• Particulate silica (.xlsx)
• Particulate carbon and nitrogen concentrations (.xlsx)
• Low level nitrogen concentrations (.xlsx)
• 14C photosynthetron (.xlsx)
• Flow cytometry cell abundances (.xlsx)
• 14C primary productivity arrays (all .xlsx)
• Array 1
• Array 2
• Array 3
• Array 4
• Array 5
• Sediment trap PC/PN flux/strong (.xls)
• UPDATED! CMORE Summer 2015 Diversity Samples (19MB .xlsx)

Laboratory Protocols

• High sensitivity nitrate concentrations (.docx)
• Determination of particulate phosphorus concentrations (.doc)
• Measurement of primary production (.docx)
• Measurement of bacterial production (.docx)
• ATP concentrations (.doc)
• Silicic acid concentrations (.doc)
• Phosphate concentrations (.doc)
• Diversity Group Protocols (PDF)
• Boström, et al., 2004. “Optimization of DNA extraction for quantitative marine bacterioplankton community analysis” (PDF)
• Brody & Kern, 2004. “History and principles of conductive media for standard DNA electrophoresis” (PDF)
• Brody, et al., 2004. “Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media” (PDF)
• Caporaso, et al., 2012. “Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms” (PDF)
• Miller, et al., 1988. “A simple salting out procedure for extracting DNA from human nucleated cells” (PDF)
• Mueller, et al., 2015. “Variables Influencing Extraction of Nucleic Acids from Microbial Plankton (Viruses, Bacteria, and Protists) Collected on Nanoporous Aluminum Oxide Filters” (PDF)
• Steward & Culley, 2010. “Extraction and purification of nucleic acids from viruses” (PDF)
• Watson, et al., 1998 “A New Method for DNA and RNA Purification” (PDF)
• Chavez, et al., 1995. “On the Chlorophyll a Retention Properties of Glass-Fiber GF/F Filters” (PDF)
• Karl, 2007. “Plastics-Irradiated-Etched: The Nuclepore® Filter Turns 45 Years Old” (PDF)
• “Membrane filtration: A User’s Reference” and “Polycarbonatemembrane filters” (PDF)
• Burford and Pollard, 1994. “Pigment contaminants in polycarbonate filters” (PDF)

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