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HOT Program

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Time at Sea
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Time at Sea  
The highlights of the course were two one-week cruises aboard the R/V Kilo Moana. These cruises provided students with a first hand look at modern oceanographic techniques. In addition, teams of students conducted a series of experiments using the tools and concepts covered in the daily labs and lectures. The cruises were designed to expose students to a broad array of instruments and methods available to oceanographers for sensing microbes in the sea. Several of the course faculty participated in the cruises, providing instruction and guidance in hypotheses testing, sampling, and experimental design. Karin fixing oxygen sample
While at sea, students shared cabins. All linens were provided on the research vessel. In addition, three daily meals were served at sea. Although the R/V Kilo Moana is renowned for its stability at sea, students prone to seasickness or those unsure if they get seasick were advised plan to bring appropriate medication. Students are also advised to plan to bring a pair of closed-toed shoes that could get wet to wear while sampling and working on the ship, and to bring another pair of shoes or sandals (that don’t get wet) to wear around the ship when not working.
CTD Sampling

Experiments at Sea:

  • In situ incubations
  • Photosynthesis and optics
  • Particulate matter concentration
  • Nutrient concentration

UH Labs

  • Primary production
  • Nutrient analyses
  • Respiration
  • Bacterial production
  • Flow cytometry
  • Microscopy
  • ATP concentration


  • Chlorophyll concentration
  • DNA/RNA extraction
  • PCR
  • Cloning
  • Screening
  • Bioinformatics
  • Modeling


Last updated Mon 09 Apr 07.