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2013 Cruise Data and Laboratory Protocols
Data from the 2013 Kilo Moana cruise
(Tuesday, June 11 thru Friday, June 21
Please note: As these data sets and protocols become available after the cruise, access will be restricted to Summer Course participants and login will be required. Mahalo!
Data Sets
- Cruise Binder (PDF)
- CTD Data (FTP)
- Underway Data (FTP)
- Surface PAR Data (FTP)
- Surface PAR Plot (PDF)
- AA nutrients (Excel .xlsx)
- ATP cruise data (Excel .xlsx)
- Fluorometric chlorophyll (Excel .xls)
- Primary production arrays (Excel .xlsx)
- Bio-optical measurements
- a_dissolved.csv
- a_particle.csv
- c_dissolved.csv
- c_particle.csv
- kdpar.csv
- parpercent.csv
- scaled_Ed.mat
- Particulate phosphorus (Excel .xls)
- Water column PC/PN (Excel .xlsx)
- Bacterial Production Array Measurements (Excel .xlsx)
- Nutrient summary (Excel .xls)
- PC/PN trap fluxes (Excel .xlsx)
- Dissolved silicate (Excel .xlsx)
- Particulate silica (Excel .xlsx)
Laboratory Protocols
- High sensitivity nitrate concentrations (Word)
- Determination of particulate phosphorus concentrations (Word)
- Nucleic acid extraction and amplification from marine bacteria (PDF)
- Measurement of primary production (Word)
- Measurement of bacterial production (Word)
- ATP concentrations (Word)
- Silicic acid concentrations (Word)
- Phosphate concentrations (Word)
- CARD-FISH: protocol (PDF) and table of probes (PDF)
New! Tag Sequencing Methods and Results
- Diversity Group Sample Data.xlsx (Excel .xlsx)
- Sample Preparation for the MiSeq Sequencing Run (PDF)
- Caporaso, et al, 2012. “Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms” (PDF)
- Watson, et al., 1998. “A New Method for DNA and RNA Purification” (PDF)
- Miller, et al., 1988. “A simple salting out procedure for extracting DNA from human nucleated cells” (PDF)
- Steward & Culley, 2010. “Extraction and purification of nucleic acids from viruses” (PDF)
- Boström, et al., 2004. “Optimization of DNA extraction for quantitative marine bacterioplankton community analysis” (PDF)
- Brody, et al., 2004. “Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media” (PDF)
- Brody & Kern, 2004. “History and principles of conductive media for standard DNA electrophoresis” (PDF)
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