Laboratory for Microbial Oceanography
in the School of Ocean and Earth Science and Technology at the University of Hawai'i at Manoa

Louie @ Palmer Station Antarctica

Luis M. "Louie" Tupas


Personal

Assistant Specialist Oceanographer, 1993 - present
Graduate Faculty, 1995 - present
Department of Oceanography
School of Ocean and Earth Science and Technology
University of Hawaii at Manoa
1000 Pope Road, Honolulu, Hi 96822
Phone: (808) 956-3311 Fax: (808) 956-9516
email: ltupas@soest.hawaii.edu

Positions held

Post-doctoral Researcher, 1991-1993
SOEST, University of Hawaii at Manoa

Lecturer, Department of Earth Sciences, 1988-1990
Japan Meteorological College

Instructor, Department of Zoology, 1984-1985
University of the Philippines, Diliman

Research Associate, Marine Sciences Institute, 1983-1985
University of the Philippines, Diliman

Education

Ph.D. Oceanography, 1991, Ocean Research Institute, The University of Tokyo

M.Sc. Oceanography, 1988, Ocean Research Institute, The University of Tokyo

Diploma, Japanese Language and Culture, 1986, The University of Tokyo

B.Sc. Marine Biology, 1983, University of the Philippines

Dissertation and Thesis

Ph.D. Dissertation: Nitrogen metabolism by heterotrophic bacterial
populations and its ecological significance in coastal marine waters

M.Sc. Thesis: Production and utilization of amino acid nitrogen in
marine coastal environments

B.Sc. Thesis: Effects of sedimentation on the growth and survival of
colonial and solitary corals

Research interests

Microbial ecology and biochemistry, biogeochemistry of carbon and nitrogen

Research Grants and Awards

The Hawaii Ocean Time-series Program: JGOFS component (Co-Principal
Investigator). NSF-Division of Ocean Sciences. 1 June 1993 - 31 July
1998.

Japanese Government Research Award for Foreign Specialists. Japanese
Ministry of Education. March 1996. Seikai National Fisheries Research
Institute, Nagasaki, Japan and Ocean Research Institute, The University
of Tokyo, Japan.

Refereed Publications

Emerson, S., P. Quay, D. Karl, C. Winn, L. Tupas and M. Landry. 1997.
Experimental determination of the carbon flux from open-ocean surface
waters. Nature (in press).

Karl, D., R. Letelier, L. Tupas, J. Dore, J. Christian, D. Hebel. 1997.
The role of nitrogen fixation in biogeochemical cycling in the
subtropical North Pacific Ocean. Nature 388: 533-538.

Dore, J.E., T. Houlihan, D.V. Hebei, G.Tien, L. Tupas and D.M. Karl.
1996.  Freezing as a method of sample preservation for the analysis of
dissolved inorganic nutrients in seawater.  Mar.  Chem. 53:173-185.

Karl, D.M., J.R. Christian, J.E. Dore, D.V. Hebel, R.M. Letelier, L.M.
Tupas and C.D. Winn.  1996.  Seasonal and interannual variability in
primary production and particle flux at Station ALOHA.  Deep-Sea Res.
43: 539-568.

Sharp, J.H., R. Benner, L. Bennett, C.A. Carlson, S.E. Fitzwater, E.T.
Peltzer and L.M. Tupas.  1995.  Analyses of dissolved organic carbon in
seawater: The JGOFS EQPAC methods comparison.  Mar.  Chem. 48: 91-108.

Karl, D.M., R. Letelier, D. Hebel, L. Tupas, J. Dore, J. Christian and
C. Winn. 1995.  Ecosystem changes in the North Pacific subtropical gyre
attributed to the 1991-92 El Nino.  Nature 373: 230-234.

Tupas, L.M., I. Koike, D.M. Karl and 0. Holm-Hansen. 1994.  Nitrogen
metabolism by heterotrophic bacterial assemblages in Antarctic coastal
waters.  Polar Biol. 14: 195-204.

Tupas, L.M., B.N. Popp and D.M. Karl. 1994.  Dissolved organic carbon
in oligotrophic waters:  experiments on sample preservation, storage
and analysis.  Mar.  Chem. 45: 207-216.

Koike, I and L.M. Tupas. 1993.  Total dissolved nitrogen in the
Northern North Pacific Ocean assessed by a high-temperature combustion
method.  Mar.  Chem. 41: 209-214.

Tupas, L.M., I. Koike and O. Holm-Hansen. 1991.  Nitrogen dynamics in
Antarctic coastal waters during a spring phytoplankton bloom (in
Japanese).  Gekkan Kaiyo (Marine Science Monthly) 23: 694-702.

Tupas, L.M. and I. Koike. 1991.  Simultaneous uptake and regeneration
of ammonium by mixed assemblages of heterotrophic marine bacteria.
Mar.  Ecol.  Prog,.  Ser. 70: 273-282.

Tupas, L.M. and I. Koike. 1990.  Amino acid and ammonium utilization by
heterotrophic marine bacteria grown in enriched seawater.  Limnol.
Oceanogr. 35: 1145-1155.

Tupas, L.M. and N. E. Montano. 1987.  Effects of aqueous alkaline
extracts from Philippine seaweeds as a foliar spray on crops.  Phil.
J. Sci. 17: 29-36.

Montano, N.E. and L.M. Tupas. 1987.  Growth response of some plants to
treatment with aqueous extracts from Sargassum polycystum and
Hydroclathrus clathratus at various pH levels.  Phil.  J. Sci. 17:
11-22.

Montano, N.E., L.M. Tupas and A.H. Luistro. 1985.  Studies on
Philippine semi-refined carageenan. 1. Water insoluble matter, total
and acid insoluble ash, and viscosity and absorbance of solutions.
Fish.  Res.  J. Phil. 10: 35-50.

Other Publications

Tupas, L.M. 1994.  Euphotic zone nitrate variability in the central
North Pacific Gyre at the Hawaii Ocean Time-series Station ALOHA.
International WOCE Newsletter 17: 21-23.

Tupas, L.M., I. Koike and 0. Holm-Hansen. 1990.  RACER: Uptake rates of
ammonium and nitrate by phytoplankton populations during the 1989
austral spring bloom.  Antarc.  J. US 25(5): 146-148.

Tupas, L.M., I. Koike and 0. Holm-Hansen. 1990.  RACER: Microbial
uptake and regeneration of ammonium during the 1989 austral spring
bloom.  Antarc.  J. US. 25(5): 154-155.

Alino, P.M., G.J.B. Cajipe, E.T. Ganzon-Fortes, W.R.Y. Licuanan, N.E.
Montano and L.M.  Tupas. 1990.  The use of marine organisms in folk
medicine and horticulture: a preliminary study.  Seaweed Information
Center, University of the Philippines.

Montano, N.E. and L.M. Tupas. 1990.  Plant growth hormonal activities
of aqueous extracts from Philippine seaweeds.  Seaweed Information
Center, University of the Philippines.

Tupas, L.M. and I. Koike. 1988.  Excretion of nitrogen compounds by
some benthic marine animals.  Ohtsuchi Mar.  Res.  Cent.  Rep. 14:
55-63.

Abstracts

Tupas, L.M. 1996. Deep-sea biogeochemical fluxes at US-JGOFS
time-series Station ALOHA. Fifth MASFLECS Symposium. Nagasaki, Japan.

Hebel, D.V., D.M. Karl, J.R. Christian, J.E. Dore, R.M. Letelier, L.M.
Tupas and C.D. Winn. 1996. Seasonal and interannual variability in
primary production and particle flux at Station ALOHA. EOS 76: OS85.

Tupas, L.M., M.P. Sampson and D.M. Karl. 1996.  Stable nitrogen
isotopic analysis of sinking particulate matter at the Hawaii Ocean
Time-series site.  EOS 76: OS86.

Tupas, L.M. 1995.  Unexpected biogeochemical cycling pathways at the
US-JGOFS time series station ALOHA. 1995 Gordon Research conference on
Chemical Oceanography, New England College, Henniker, New Hampshire,
USA.

Fujieki, L.A., D.V. Hebel, L.M. Tupas and D.M. Karl. 1994.  Hawaii
Ocean Time-series data organization and graphical system (HOT-DOGS).
The Oceanography Society Pacific Basin Meeting.  Honolulu, Hawaii,
USA.

Houlihan, T., J. Dore, L. Tupas, D. Hebel, G. Tien and D Karl. 1994.
Freezing as a method of preservation for seawater dissolved nutrient
and organic carbon samples.  The Oceanography Society Pacific Basin
Meeting.  Honolulu, Hawaii, USA.

Tupas, L., B. Popp and D. Karl. 1994.  Dissolved organic carbon
measurements at Station ALOHA by high temperature catalytic oxidation:
Characteristics and variation in the water column.  The Oceanography
Society Pacific Basin Meeting.  Honolulu, Hawaii, USA.

Sharp, J.H., R. Benner, L. Bennett, C.A. Carlson, S.E. Fitzwater, E.T.
Peltzer and L.M. Tupas.  1993.  The Equatorial Pacific intercalibration
analyses of dissolved organic carbon in seawater.  Joint Meeting of the
American Society of Limnology and Oceanography and Society of Wetland
Scientists.  Edmonton, Alberta, Canada.

Sharp, J.H., R. Benner, L. Bennett, C.A. Carlson, S.E. Fitzwater, E.T.
Peltzer and L.M. Tupas.  1993.  Dissolved organic carbon:
intercalibration analyses with Equatorial Pacific samples.  Third
Scientific Meeting of The Oceanography Society, Seattle, Washington,
USA.

Tupas, L.M., B.N. Popp and D.M. Karl. 1992.  Dissolved organic carbon
in oligotrophic waters; experiments on sample preservation, storage and
analysis.  Fall meeting of the American Geophysical Union, San
Francisco, California.  EOS 73 (43): 287.

Tupas, L.M., B.N. Popp and D.M. Karl. 1992.  Preservation, storage and
analysis of samples for the determination of dissolved organic carbon
in seawater. 1992 Gordon Research Conference on Organic Geochemistry,
Colby-Sawyer College, New London, New Hampshire, USA.

Tupas, L.M. and I. Koike. 1991.  Carbon and nitrogen fluxes in
Antarctic coastal waters.  Symposium on Polar Studies, Fall Meeting of
the Oceanographical Society of Japan, Tokyo,Japan.

Tupas, L.M. and I. Koike. 1989.  Utilization of ammonium and dissolved
organic nitrogen by mixed populations of heterotrophic marine
bacteria.  Fifth International Congress on Microbial Ecology, Kyoto,
Japan.

Tupas, L.M. and I. Koike. 1988.  Microbial utilization of amino acids
from Mytilus edulis.  Fall meeting of the Oceanographical Society of
Japan, Tokyo, Japan.

Tupas, L.M. and N.E. Montano. 1985.  Growth hormone-like extracts from
various marine macro- algae.  First Philippine-US Phycology Workshop,
Manila, Philippines.

Technical Reports

Karl, D., L. Tupas, F. Santiago-Mandujano, C. Nosse, D. Hebel, E.
Firing and R. Lukas. 1996 Hawaii Ocean Time-series Program: Data Report
7: 1995. University of Hawaii, School of Ocean and Earth Science and
Technology Technical Report 96-09. 228 pages.

Tupas, L.M., F. Santiago-Mandujano, D. Hebel, E. Firing, R. Lukas and
D. Karl. 1994.  Hawaii Ocean Time-series Program: Data Report 6: 1994.
University of Hawaii, School of Ocean and Earth Science and Technology
Technical Report 95-06. 199 pages.

Tupas, L.M., F. Santiago-Mandujano, D. Hebel, E. Firing, F. Bingham, R.
Lukas and D. Karl.  1994.  Hawaii Ocean Time-series Program: Data
Report 5: 1993.  University of Hawaii, School of Ocean and Earth
Science and Technology Technical Report 94-05. 156 pages.

Tupas, L.M., F. Santiago-Mandujano, D. Hebel, R. Lukas, D. Karl and E.
Firing. 1993.  Hawaii Ocean Time-series Program: Data Report 4: 1992.
University of Hawaii, School of Ocean and Earth Science and Technology
Technical Report 93-14. 248 pages.

Tupas, L.M. and I. Koike. 1991.  Nitrogen utilization by water column
bacterial populations in the Western North Pacific Ocean (in
Japanese).  In: I. Koike (ed.) Preliminary report of the Hakuho-Maru
cruise KH 91-3, JGOFS Western North Pacific Ocean.  Ocean Research
Institute, The University of Tokyo.

Tupas, L.M. and I. Koike. 1991.  Carbon and nitrogen analysis of
sub-micron particles in waters of the Western North Pacific Ocean (in
Japanese).  In: I. Koike (ed.) Preliminary report of the Hakuho-Maru
cruise KH 91-3, JGOFS Western North Pacific Ocean.  Ocean Research
Institute, The University of Tokyo.

Tupas, L.M. and I. Koike. 1991.  Dissolved amino acid concentrations in
the water column of the Western North Pacific Ocean (in Japanese).  In:
I. Koike (ed.) Preliminary report of the Hakuho-Maru cruise KH 91-3,
JGOFS Western North Pacific Ocean.  Ocean Research Institute, The
University of Tokyo.

Tupas, L.M. and I. Koike. 1987.  Excretion of organic and inorganic
material by microzooplankton and the utilization of excreta by
heterotrophic marine bacteria in the North Pacific Ocean.  In: A.
Hattori (ed.) Preliminary report of the Hakuho-Maru cruise KH 86-3,
Bering Sea and Northern North Pacific Ocean.  Ocean Research Institute,
The University of Tokyo.

Tupas, L.M. and I. Koike. 1987.  Vertical distribution of total
dissolved nitrogen in the Northern North Pacific Ocean.  In: A. Hattori
(ed.) Preliminary report of the Hakuho-Maru cruise KH 86-3, Bering Sea
and Northern North Pacific Ocean.  Ocean Research Institute, The
University of Tokyo.

Tupas, L.M. and I. Koike. 1987.  Growth and carbon conversion
efficiencies of bacterial populations in the Northern North Pacific
Ocean.  In: A. Hattori (ed.) Preliminary report of the Hakuho-Maru
cruise KH 86-3, Bering Sea and Northern North Pacific Ocean.  Ocean
Research Institute, The University of Tokyo.

Reviewer

Limnology and Oceanography
National Science Foundation, 
Division of Ocean Sciences
Division of Environmental Biology
Division of Polar Program

Membership in Scientific Organizations

American Society of Limnology and Oceanography
American Geophysical Union
American Society of Microbiology
Oceanographical Society of Japan
Japanese Society of Microbial Ecology