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CTD PROTOCOLS AND SALINITY

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     SUMMARY:  A commercially-available instrument package is 
     lowered into the ocean on a conducting cable to obtain real-
     time, high-resolution profiles of temperature, conductivity 
     and pressure from which salinity and depth are calculated.  
     Additional sensors also detect dissolved oxygen concentration 
     and phytoplankton fluorescence.  Water samples are collected
     in bottles attached to the rosette sampler which can be
     activated by a surface control unit.
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1.  Principle

    High vertical resolution environmental data are collected with 
a SeaBird CTD having external temperature (T), conductivity (C) and 
dissolved oxygen (DO) sensors and an internal pressure sensor.  There 
is also a provision for adding fluorescence (F) and other sensors.  
A General Oceanics 24-place pylon and an aluminum rosette containing 
24 12-liter Niskin bottles is used to obtain water samples from 
predetermined depths.  The CTD and rosette are deployed on a 3-conductor 
cable allowing for the real-time display of data and acquisition, and 
for tripping the bottles in areas of interest in the water column.  
The CTD system takes 24 samples per second of pressure (P), T, C, DO 
and F.  The raw data are stored both on the computer and, for redundancy, 
on VHS-format video tapes. 

2. CTD Data Collection  

2.1. CTD data are taken with a SeaBird SBE-09 CTD having internal 
     pressure sensor, and external temperature, conductivity and 
     dissolved oxygen sensors.    
   
2.2. The CTD is mounted in an aluminum Scripps-type rosette with 24 
     places for Niskin bottles.  Water samples are taken on the upcast 
     for calibration of the conductivity and oxygen sensors.  

2.3. CTD data are recorded during both down and up casts.  When the 
     Niskin bottles are tripped, an event mark is made in the data 
     files to record the time and CTD data.

2.4. One deep cast to approximately 4700 m (total water depth = 4750 m) 
     is made on each cruise.

2.5. Ten to twelve consecutive casts to 1000 m are made over 36 hours 
     to span the local inertial period (~31 hour) and three semi-diurnal 
     tidal cycles.  This sampling is performed so that tidal or near-
     inertial variability can be estimated and removed, thereby 
     preventing an aliasing of the data records by these components.  
     During this 36 hour intensive sampling period, most of the water 
     samples for the GOFS program objectives are collected.

2.6. In 1989, SeaBird introduced a ducting arrangement which is designed 
     to minimize salinity spiking.  This "T-C" duct has been used in 
     cruises since HOT-11.

3.  CTD Sensors and Calibrations

3.1. Pressure:  CTD pressure is measured with a 6,000 dbar 
     Paroscientific Digiquartz pressure transducer with an internal 
     temperature sensor.  Pressure calibrations are conducted twice 
     yearly against a reference Paroscientific pressure standard 
     using a dead weight pressure tester to impose test pressures.  
     The pressure standard is recalibrated at the Northwest Regional 
     Calibration Center (NWRCC) every two years.  Pressure is 
     corrected for thermal shock effects using a linear response 
     method (Chiswell, 1990):

                          P = aPm + h * T + b

     where: *= temporal convolution operator
            h= impulse response function
            T= water temperature
           Pm= measured pressure

     The a and b calibration coefficients are determined from a 
     calibration at constant temperature and pressure.  The impulse 
     response function, h, is computed by measuring pressure 
     perturbations when the CTD is plunged into a cold water bath.

3.2. Temperature sensor:  CTD temperature sensors can be removed and 
     calibrated independently.  The SeaBird model SBE-3-02/F temperature 
     sensors are calibrated annually at the NWRCC.  A check on the 
     temperature sensors is maintained by intercomparing our pool of 
     sensors between each cruise.  Two sensors (#741 and #866) were 
     used during 1989.  These sensors were calibrated at Northwest 
     Regional Calibration Center prior to HOT-1, and again in November 
     1989.  Between each cruise, they were compared against each other 
     and against two additional sensors acquired in August 1989.  These 
     intercomparisons allowed us to map sensor drift that occurred 
     between the NWRCC calibrations.  Each HOT program data report 
     will provide a Table of temperature sensor corrections for the 
     period of investigation.
3.3. Conductivity sensor:  The SeaBird model SBE-4 conductivity cell 
     is calibrated by comparing CTD-recorded conductivities with 
     conductivities computed from the discrete water samples.  At 
     least three samples are used per cast; salinity minimum, 
     salinity maximum and mixed layer.  On three casts, together 
     comprising the "WOCE deep-cast," much higher vertical resolution 
     sampling of the salinity is done to get the best possible 
     salinity profile.  

3.4. Dissolved oxygen (DO) sensor:  A SeaBird model SBE-13 dissolved 
     oxygen sensor is used employing a polargraphic sensor, 
     manufactured by SensorMedics.  The DO sensor consists of a 
     teflon membrane covering a layer of KCl  gel.  A constant 
     voltage applied across two electrodes results in a current
     nearly proportional to the activity of oxygen diffusing across 
     the membrane.  This current and the temperature of the cell 
     are measured, and DO is calculated using an algorithm based on 
     Owens and Millard (1985):

     DO = (a1 OC + a2) OSAT (P,T,S) exp (a3 T + a4 OT + a5 p + a6 dOC/dt)

     where: OC= sensor current
            OT= sensor temperature
          OSAT= saturation concentration of oxygen 
             P = pressure
             T = temperature
             S = salinity

     The coefficients a1,....,a6 are determined from a nonlinear 
     least-squares fit against check samples taken from bottles 
     during the deep cast.  Because the DO sensor shows considerable 
     hysteresis, the calibration is made using the down cast values 
     of OC, OT, P, T, S at the same density levels of the bottles.

3.5. Fluorescence sensor:  Stimulated fluorescence is measured using 
     a Sea-Tech flash fluorometer with an excitation wavelength of 
     425 nm peak emission and a peak response at 685 nm (30 nm FWHM).

4.  Salinity Determinations

    Salinity samples are collected directly from the Niskin bottles 
    into 250 ml polyethylene bottles and stored at room temperature, 
    in the dark, for subsequent analysis at our shore-based laboratory.  
    The time between sample collection and analysis is generally less 
    than 1 week.  Prior to analysis, the samples are equilibrated to 
    laboratory temperature and the salinity measured using an AGE 
    model 2100 Minisal salinometer which is calibrated against IAPSO 
    standard (Wormley) seawater.  Typical precision of replicate 
    analyses from the same water sample is 0.0003 o/oo; for triplicate 
    sample bottles the precision is less than 0.001 o/oo.  The effects 
    of sample storage in polyethylene bottles was systematically 
    evaluated during year 1 of the HOT program.  The data, summarized 
    in the 1988-89 HOT Data Report, indicate that S o/oo changes were 
    negligible.

5.  CTD Post-processing

    Once the CTD data are collected and laboratory-determined pressure 
and temperature calibrations are applied, they are subjected to 
screening and quality control; they are checked for spikes or missing 
data caused by electrical interference in the hydrowire.  Spikes are 
removed with a 9-point median filter, and missing data are replaced 
with interpolated values.  After this initial screening, the data 
are averaged to 1/2-second values.  Pressure and conductivity are 
then corrected for thermal hysterisis effects.  This processing in 
the time-domain is required to allow for correction of lags between 
the C- and T- sensor responses.  After the data have been reduced to 
1/2-second values, corrections derived from the calibration methods 
described above are applied to the conductivity and dissolved oxygen.  
Finally, the data are pressure-sorted to remove effects of shiproll 
(i.e., only data taken when the CTD is moving downwards are kept), 
and averaged into 2 decibar values.

6.  References

    Chiswell, S. M.  Dynamic reaponse of SeaBird CTD pressure sensors 
    to temperature.  In prep.

    Owens, W. B. and R. C. Millard, Jr.  1985.  A new algorithm for 
    CTD oxygen calibration.  Journal of Physical Oceanography, 15, 
    621-631.