Hawaii Ocean Time-series (HOT)
in the School of Ocean and Earth Science and Technology at the University of Hawai'i

Chloropigment by Flash Fluorescence

Seapoint Fluorometer

Seapoint

FTP View Data
To assist in the interpretation of the data, it can be displayed using the Hawaii Ocean Time-series Data Organization & Graphical System (HOT-DOGS©).

Analytical Method

Fluorescence was measured with a Sea Tech Model ST0250 flash fluorometer (#32) up until HOT-130, then with a Sea-Point chlorophyll fluorometer (#2440 and #2441) from HOT-131 onwards. The data was collected using the Sea-Bird CTD system. Fluorescence traces were collected on as many casts as possible. Because an absolute radiometric standard is not available for fluorometers, instrument drift was corrected via calibration with bottle fluorometric chlorophyll a plus accessory pheopigments analyzed using a Turner Designs Model 10-AU fluorometer. A linear relationship of the form Vchl = b*Vfluor +a was used to convert all fluorescence data to chloropigment.

Results

Stack plots of chloropigment (fluorescence) and bottle fluorometric chlorophylls+pheopigments for 2015 are shown in the figures below. The offset is .2 µg/l.


Chloropigment profiles show the chlorophyll maximum at the base of the euphotic zone, characteristic of the central North Pacific Ocean. Chloropigment profiles show the influence of internal waves when plotted againt pressure, but remain relatively constant within a cruise when plotted against potential density (σθ). However, there is substantial cruise-to-cruise variability in both the position and magnitude of the chlorophyll maximum.

Representative chloropigment profiles plotted against pressure for a period of 27 years are shown in Figure 4A, Figure 4B, Figure 4C, Figure 4D & Figure 4E and against potential density in Figure 5A, Figure 5B, Figure 5C, Figure 5D & Figure 5E. In order to facilitate comparison, only night-time profiles are presented after normalization to the average density profile obtained from the CTD burst sampling for each cruise. Month-to-month variability in the average depth of the chlorophyll maximum is apparent.