Discrete cruise measurements/experiments
1. Sampling during selected HOE-DYLAN cruises
- Picoeukaryote population dyamics measured via CTD profiles and deckboard incubation
experiments (Shimi Rii; KM1215)
- Measuring the abundance, diversity, distribution, and gene expression of UCYN-A and
its associate at Station ALOHA (Anne Thompson; KM1219)
- Metabolic patterns of eukaryotic phytoplankton and Trichodesmium using
metatranscriptome profiling (Sonya Dyhrman, Monica Rouco Molina, Harriet Alexander;
KM1217, KM1219)
- Study of the abundance and distribution of clades I and II of the nitrogen-fixing
cyanobacterium Trichodesmium sp. with depth and time (Monica Rouco Molina; KM1217)
- Hydrogen cycling associated with nitrogen fixation (Sam Wilson; KM1215, KM1219)
- Metabolism of DMSP, specifically the effect of availability of substrate and C1-compounds.
I am also interested in looking at the potential competition between Prochlorococcus
and heterotrophic bacteria (especially SAR11) for DMSP and methionine. I will also be
conducting experiments related to methane production from methylphosphonic acid
(Daniela del Valle; KM1219)
- Nutrient addition experiments to investigate factors regulating the utilization of DOM by
heterotrophic bacteria (Sandra Martinez-Garcia; KM1219)
- Completing an intercalibration of sample collection methods for colloidal Fe using
microfiltration and cross-flow filtration techniques (Jess Fitzsimmons; KM1215)
- Analysis of low molecular weight acids and photorespiration during incubation experiments
and daily profiles (John Casey; KM1219)
- Analysis of soluble and colloidal Fe-binding ligands by cross flow filtration. The
samples will be analyzed by HPLC-ICPMS in order to separate and identify Fe-binding ligands
across Fe size fractions (Rene Boiteau; KM1219)
- Thorium isotopes depth profile for quantification of metal scavenging (Jess Fitzsimmons,
Katharina Pahnke; KM1215)
- Fe isotope profile (Jess Fitzsimmons; KM1215)
- Screening isolated microbes for reactive oxygen species (ROS) production
(Emily Estes; KM1215)
- Role of ROS, particularly superoxide in the water column. Measuring concentration,
decay rates, and production (photochemical and biological) of superoxide (Kelly Roe;
KM1215)
- Characterize biological production and decay rates of hydrogen peroxide under both
light and dark conditions (Robin Schneider; KM1215)
- Measuring the gene expression of size-fractionated microbial communities at 25 m,
DCM and 250 m (Kristina Fontanez; KM1219)
- Response of Prochlorococcus populations to intermittent nitrate upwelling (Paul Berube;
KM1215, KM1217, and KM1219)
2. Deckboard incubation experiments
- Remineralization capabilities of heterotrophic communities at different depths by deckboard
incubation studies (Brian Burkhardt; KM1215)
- Comparison of carbon and nitrogen fixation rates by Trichodesmium colonies under
various carbon dioxide, light and nutrient conditions (Rosie Gradoville; KM1217)
- Characterization of the Trichodesmium phylotypes in colonies collected by net tows
(Rosie Gradoville; KM1217)
- Nitrogen addition experiment to determine the response of photosynthetic picoeukaryote
assemblage structure and physiology to variations in nitrogen concentrations and sources
(Shimi Rii; KM1215)
- Ocean acidification experiments (Daniela Böttjer/Donn Viviani; KM1219)
- Nutrient-pertubation experiments to examine the response of the phytoplankton assemblages
in near-surface waters to different N species (Kendra Turk-Kubo; KM1219)
- Photosynthesis-Irradiance Curves, Day length manipulation experiments, FCM determination
of fluorescence to scattering ratios as a function of growth irradiance (Angelique
White/Katie Watkins-Brandt/Morgaine McKibben; KM1217 & KM1219)
- POM-enrichment experiment to examine gene expression of particle-associated surface
communities (Kristina Fontanez; KM1219)
- Nitrification rate measurements associated with the NO2 maximum and using
inhibitors for bacteria and archaea (Alex Galan; KM1219)
3. Miscellaneous
- ALOHA profiler Communicate with the ALOHA profiling mooring
- Satellite calibration Matching VIIRS data (Curtiss Davis, Ricardo Letelier),
with HPLC data (Bob Bidigare), HyperPRO (Benedetto/Angel)
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